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Convergence of Ubiquitylation and Phosphorylation Signaling in Rapamycin-Treated Yeast Cells

ABSTRACT: Protein, Ubiquitylation, and Phosphorylation changes were quantified after 1 and 3 hours of rapamycin treatment in budding yeast. Approximately 3,600 proteins, 9,000 phosphorylation sites, and 2,500 ubiquitylation sites were quantified.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Saccharomyces Cerevisiae (baker's Yeast)

SUBMITTER: Brian Weinert

LAB HEAD: Chunaram Choudhary

PROVIDER: PXD000554 | Pride | 2014-10-03

REPOSITORIES: Pride

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Convergence of ubiquitylation and phosphorylation signaling in rapamycin-treated yeast cells.

Iesmantavicius Vytautas V Weinert Brian T BT Choudhary Chunaram C

Molecular & cellular proteomics : MCP 20140624 8

The target of rapamycin (TOR) kinase senses the availability of nutrients and coordinates cellular growth and proliferation with nutrient abundance. Inhibition of TOR mimics nutrient starvation and leads to the reorganization of many cellular processes, including autophagy, protein translation, and vesicle trafficking. TOR regulates cellular physiology by modulating phosphorylation and ubiquitylation signaling networks; however, the global scope of such regulation is not fully known. Here, we us ...[more]

PMID: 24961812

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