Proteomic analysis of Pichia pastoris GS115

ABSTRACT: Pichia pastoris GS115 was cultured in YPD medium and protein were extracted and separated on SDS-PAGE followed by in-gel trypsin digestion. Protein were also digested in-solution using trypsin and fractionated using SCX and bRPLC. A total of 101 peptide fractions were analyzed on LTQ-Orbitrap Velos mass spectrometer. The raw data was searched using Mascot, Sequest and MS Amanda search algorithms against P. pastorisGS115 NCBI RefSeq database through Proteome Discoverer software suite.

INSTRUMENT(S):

ORGANISM(S): Komagataella Pastoris (strain Gs115 / Atcc 20864) (yeast) (pichia Pastoris)

SUBMITTER: Akhilesh Pandey  

LAB HEAD: Akhilesh Pandey

PROVIDER: PXD000627 | Pride | 2014-11-18

REPOSITORIES: Pride