CSF proteome mapping: Human cerebrospinal fluid LC-MS/MS, Part 1

ABSTRACT: 3 mL CSF from a pool of 21 neurologically healthy donors was separated into a bound and a depleted fraction using a MARS Hu-14 column. This experiment represents the bound fraction. Gel separation on a gradient gel (SDS-PAGE) followed and the gel lane was cut into 37 fractions. Each fraction was in-gel trypsin digested and analyzed separately by LC-MS/MS on an Orbitrap Velos Pro mass spectrometer. Resulting data was searched using SeachGui, summarized in PeptideShaker and exported to CSF-PR.

REANALYSED by: PAe005113

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Cerebrospinal Fluid

SUBMITTER: Astrid Guldbrandsen  

LAB HEAD: Astrid Guldbrandsen

PROVIDER: PXD000651 | Pride | 2014-08-04

REPOSITORIES: Pride