Proteomics

Dataset Information

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CSF proteome mapping: Human cerebrospinal fluid LC-MS/MS, Part 4

ABSTRACT: 1.6 mL CSF from a pool of 21 neurologically healthy donors was separated into a bound and a depleted fraction using a MARS Hu-14 column. This experiment represents the depleted fraction. Sample was in-solution trypsin digested and resulting peptides were fractionated into 66 fractions by mixed-mode reversed phase anion exchange (MM(RP-AX) using a Promix MP column. Each fraction was analyzed separately by LC-MS/MS on an Orbitrap Velos Pro mass spectrometer. Resulting data was searched using SeachGui, summarized in PeptideShaker and exported to the CSF-PR.

REANALYSED by: PAe005111

INSTRUMENT(S): LTQ Orbitrap, instrument model

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Cerebrospinal Fluid

SUBMITTER: Astrid Guldbrandsen  

PROVIDER: PXD000654 | Pride | 2014-08-04

REPOSITORIES: Pride

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Publications

In-depth characterization of the cerebrospinal fluid (CSF) proteome displayed through the CSF proteome resource (CSF-PR).

Guldbrandsen Astrid A   Vethe Heidrun H   Farag Yehia Y   Oveland Eystein E   Garberg Hilde H   Berle Magnus M   Myhr Kjell-Morten KM   Opsahl Jill A JA   Barsnes Harald H   Berven Frode S FS  

Molecular & cellular proteomics : MCP 20140718 11

In this study, the human cerebrospinal fluid (CSF) proteome was mapped using three different strategies prior to Orbitrap LC-MS/MS analysis: SDS-PAGE and mixed mode reversed phase-anion exchange for mapping the global CSF proteome, and hydrazide-based glycopeptide capture for mapping glycopeptides. A maximal protein set of 3081 proteins (28,811 peptide sequences) was identified, of which 520 were identified as glycoproteins from the glycopeptide enrichment strategy, including 1121 glycopeptides  ...[more]