CSF proteome mapping: Human plasma LC-MS/MS, Part 7
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ABSTRACT: 40 µL plasma from a pool of 20 neurologically healthy donors was separated into a bound and a depleted fraction using a MARS Hu-14 column. This experiment represents the depleted fraction. Sample was in-solution trypsin digested and peptides were fractionated into 67 fractions by mixed-mode reversed phase anion exchange (MM(RP-AX) using a Promix MP column. Each fraction was analyzed separately by LC-MS/MS on an Orbitrap Velos Pro mass spectrometer. Resulting data was searched using SeachGui, summarized in PeptideShaker and exported to the CSF-PR.
INSTRUMENT(S):
ORGANISM(S): Homo Sapiens (human)
TISSUE(S): Blood Plasma
SUBMITTER:
Astrid Guldbrandsen
LAB HEAD: Astrid Guldbrandsen
PROVIDER: PXD000657 | Pride | 2014-08-04
REPOSITORIES: Pride
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