Proteomics

Dataset Information

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PCT-SWATH kidney tissues - Rapid mass spectrometric conversion of tissue biopsy samples into permanent quantitative digital proteome maps

ABSTRACT: DDA and SWATH analysis of human kidney tissues. Data set used for PCT-SWATH paper. PCT-SWATH provides the first method to generate a digital map representing the proteome of biopsy level clinical samples from which thousands of proteins can be accurately quantified with a high degree of reproducibility across sample sets.

INSTRUMENT(S): TripleTOF 5600

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Epithelial Cell, Kidney

DISEASE(S): Kidney Cancer

SUBMITTER: Tiannan Guo  

LAB HEAD: Ruedi Aebersold

PROVIDER: PXD000672 | Pride | 2015-02-04

REPOSITORIES: Pride

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Publications

Rapid mass spectrometric conversion of tissue biopsy samples into permanent quantitative digital proteome maps.

Guo Tiannan T   Kouvonen Petri P   Koh Ching Chiek CC   Gillet Ludovic C LC   Wolski Witold E WE   Röst Hannes L HL   Rosenberger George G   Collins Ben C BC   Blum Lorenz C LC   Gillessen Silke S   Joerger Markus M   Jochum Wolfram W   Aebersold Ruedi R  

Nature medicine 20150302 4

Clinical specimens are each inherently unique, limited and nonrenewable. Small samples such as tissue biopsies are often completely consumed after a limited number of analyses. Here we present a method that enables fast and reproducible conversion of a small amount of tissue (approximating the quantity obtained by a biopsy) into a single, permanent digital file representing the mass spectrometry (MS)-measurable proteome of the sample. The method combines pressure cycling technology (PCT) and seq  ...[more]

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