Ontology highlight
ABSTRACT:
INSTRUMENT(S): LTQ Orbitrap Velos, Q Exactive
ORGANISM(S): Homo Sapiens (human)
SUBMITTER: Vackar Afzal
LAB HEAD: Prof Angus Lamond
PROVIDER: PXD000678 | Pride | 2014-03-12
REPOSITORIES: Pride
eLife 20140101
Technological advances have enabled the analysis of cellular protein and RNA levels with unprecedented depth and sensitivity, allowing for an unbiased re-evaluation of gene regulation during fundamental biological processes. Here, we have chronicled the dynamics of protein and mRNA expression levels across a minimally perturbed cell cycle in human myeloid leukemia cells using centrifugal elutriation combined with mass spectrometry-based proteomics and RNA-Seq, avoiding artificial synchronization ...[more]