Proteomics

Dataset Information

68

A Proteomic Chronology of Gene Expression through the Cell Cycle in Human Myeloid Leukemia Cells.


ABSTRACT: Technological advances have enabled the analysis of cellular protein and RNA levels with unprecedented depth and sensitivity, allowing for an unbiased re-evaluation of gene regulation during fundamental biological processes. Here, we have chronicled the dynamics of protein and mRNA expression levels across a minimally perturbed cell cycle in human myeloid leukemia cells using centrifugal elutriation combined with mass spectrometry-based proteomics and RNA-Seq, avoiding artificial synchronization procedures. We identify myeloid-specific gene expression and variations in protein abundance, isoform expression and phosphorylation at different cell cycle stages. We dissect the relationship between protein and mRNA levels for both bulk gene expression and for over ~6,000 genes individually across the cell cycle, revealing complex, gene-specific patterns. This dataset, one of the deepest surveys to date of gene expression in human cells, is presented in an online, searchable database, the Encyclopedia of Proteome Dynamics (http://www.peptracker.com/epd/).

INSTRUMENT(S): LTQ Orbitrap Velos, Q Exactive

ORGANISM(S): Homo sapiens  

TISSUE(S): Tissue Not Applicable To Dataset

DISEASE(S): Not Available

SUBMITTER: Vackar Afzal  

LAB HEAD: Prof Angus Lamond

PROVIDER: PXD000678 | Pride | 2014-03-12

REPOSITORIES: Pride

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Publications

A proteomic chronology of gene expression through the cell cycle in human myeloid leukemia cells.

Ly Tony T   Ahmad Yasmeen Y   Shlien Adam A   Soroka Dominique D   Mills Allie A   Emanuele Michael J MJ   Stratton Michael R MR   Lamond Angus I AI  

eLife 20140101


Technological advances have enabled the analysis of cellular protein and RNA levels with unprecedented depth and sensitivity, allowing for an unbiased re-evaluation of gene regulation during fundamental biological processes. Here, we have chronicled the dynamics of protein and mRNA expression levels across a minimally perturbed cell cycle in human myeloid leukemia cells using centrifugal elutriation combined with mass spectrometry-based proteomics and RNA-Seq, avoiding artificial synchronization  ...[more]

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