Ontology highlight
ABSTRACT:
INSTRUMENT(S): LTQ Orbitrap
ORGANISM(S): Homo Sapiens (human)
SUBMITTER: Clifford Young
LAB HEAD: Ole Nørregaard Jensen
PROVIDER: PXD000686 | Pride | 2014-03-06
REPOSITORIES: Pride
Klimovskaia Ilnaz M IM Young Clifford C Strømme Caroline B CB Menard Patrice P Jasencakova Zuzana Z Mejlvang Jakob J Ask Katrine K Ploug Michael M Nielsen Michael L ML Jensen Ole N ON Groth Anja A
Nature communications 20140306
During DNA replication, nucleosomes are rapidly assembled on newly synthesized DNA to restore chromatin organization. Asf1, a key histone H3-H4 chaperone required for this process, is phosphorylated by Tousled-like kinases (TLKs). Here, we identify TLK phosphorylation sites by mass spectrometry and dissect how phosphorylation has an impact on human Asf1 function. The divergent C-terminal tail of Asf1a is phosphorylated at several sites, and this is required for timely progression through S phase ...[more]