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LC-MS/MS to identify proteins present in apoplastic fluid extracted from WT and ca1ca4 mutant Arabidopsis plants grown under low and high CO2 conditions

ABSTRACT: geLC-MS/MS analysis was performed to identify proteins that are present in apoplastic fluid isolated from rosette leaves of 8-week-old wild type and carbonic anhydrase (ca1ca4) mutant Arabidopsis. Plants were grown in either low (150 ppm) or high (500 ppm) CO2.

OTHER RELATED OMICS DATASETS IN: E-MTAB-4447PRJNA218542

INSTRUMENT(S): LTQ Orbitrap

ORGANISM(S): Arabidopsis Thaliana (mouse-ear Cress)

TISSUE(S): Plant Cell, Leaf

SUBMITTER: Jeff Anderson

LAB HEAD: Scott C Peck

PROVIDER: PXD000693 | Pride | 2014-08-22

REPOSITORIES: Pride

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Carbonic anhydrases, EPF2 and a novel protease mediate CO2 control of stomatal development.

Engineer Cawas B CB Ghassemian Majid M Anderson Jeffrey C JC Peck Scott C SC Hu Honghong H Schroeder Julian I JI

Nature 20140706 7517

Environmental stimuli, including elevated carbon dioxide levels, regulate stomatal development; however, the key mechanisms mediating the perception and relay of the CO2 signal to the stomatal development machinery remain elusive. To adapt CO2 intake to water loss, plants regulate the development of stomatal gas exchange pores in the aerial epidermis. A diverse range of plant species show a decrease in stomatal density in response to the continuing rise in atmospheric CO2 (ref. 4). To date, one ...[more]

PMID: 25043023

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LC-MS/MS of apoplast proteins from Arabidopsis wild type and ca1ca4 mutant plants

Project description:geLC-MS/MS analysiswas performed to identify proteins that are present in apoplastic fluid isolated from rosette leaves of 8-week-old wild type and carbonic anhydrase (ca1ca4) mutant Arabidopsis plants.

2014-08-22 | PXD000692 | Pride

RNA-Seq in midbrain of Coq9R239X mice treated with Rapamycin

Project description:Coq9R239X mice, a model of mitochondrial encephalopathy due to Coenzyme Q deficiency, were treated with Rapamycin administered in the chow at two different concentrations, i.e. 28 ppm and 225 ppm. The results are compared to those obtained in untreated Coq9R239X mice and untreated Coq9+/+ mice.

2019-03-21 | E-MTAB-7794 | biostudies-arrayexpress

Transcription profiling of in vivo range finding studies in the rat with selected individual food additives

Project description:FSAT01021/OBJ01/28D: TITLE: Development of methods for the assessment of the health effects from mixtures of food additives. SAP/S3 project code: 010.45073 TNO Voeding-TNO BIBRA project for UK Food Standard Agency Objective No. 01: In vivo range finding studies in the rat with selected individual food additives. Rat study using 4 compounds, oral administration in diet (concentration given in ppm), for 28 days: Butylated hydroxytoluene (BHT): 254 ppm; 762 ppm; 1523 ppm; 3046 ppm; 10154 ppm Curcumin (CC): 254 ppm; 762 ppm; 2031 ppm; 4062 ppm; 10154 ppm Propyl Gallate (PG): 203 ppm; 508 ppm; 1523 ppm; 3046 ppm; 6092 ppm Thiabendazole (TB): 102 ppm; 254 ppm; 762 ppm; 2031 ppm; 5077 ppm Within Objective No. 01: clinical chemistry, pathology, drug metabolizing enzymes are assessed as well. Within Objective No. 01: A smaller study in which rats were exposed for 3 days to two doses of BHT, CC, PG, TB was performed as well. Depending on the outcome of arrays performed within FSAT01021/OBJ01/28D, transcriptomics will be performed on these samples: the Experiment name then will be FSAT01021/OBJ01/3D for details: Rob Stierum , AVTO, Monday December 17th 2001

2008-06-09 | E-MEXP-116 | biostudies-arrayexpress

Genes expression in case of PEV caused by chromosomal rearrangement

Project description:Heterochromatic position effect variegation (PEV) is the epigenetic disruption of genes expression near the new-formed eu-heterochromatic border. We characterized the inversion In(2)A4, demonstrating cis-acting PEV as well as trans-inactivation of the reporter transgenes on the opposite normal chromosome in combination with the inversion. Euchromatic breakpoint of In(2)A4 inversion was localized at 105 bp region (chr2L:21182214-21182318) of the second exon of the Mcm10 gene, the heterochromatic breakpoint is located at the block of dodecasatellite in 2L pericentromeric heterochromatin. In order to check the effects of heterochromatin on neighbor euchromatic genes and estimate the distance of inactivation spreading, we performed RNA-seq analysis of genes expression in larvae and adults females of genotypes A12/A12 (control) and In(2)A4/In(2)A4. Cis-influence of heterochromatin in the inversion causes not only repression, but also activation of genes, and the effects of heterochromatin are different at different developmental stages. Cis-actions affect only a few genes located near the heterochromatin Comparison of genes expression in wild type and demonstrating PEV larvae and adults in two repeats each

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Human hepatocytes support the hypertrophic but not the hyperplastic response to the murine nongenotoxic hepatocarcinogen sodium phenobarbital in an in vivo study using a chimeric mouse with humanized liver

Project description:This SuperSeries is composed of the SubSeries listed below. Male CD-1 mice and WH rats (4 animals/dose) were fed diets containing 0 (control) or 2500 ppm NaPB for 7 days. Male chimeric mice (4-5 animals/dose) also fed diets containing 0 (control) or 1000 ppm NaPB for 7 days. Liver samples were used for gene expression analysis.

2014-08-29 | E-GEOD-57058 | biostudies-arrayexpress

Transcription profiling of zebra fish liver after water-borne exposure to polycyclic aromatic hydrocarbons and alkylphenols

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2008-12-11 | E-MEXP-1685 | biostudies-arrayexpress

Impacts of HLA-B57 micropolymorphism (MRM)

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2018-10-24 | PXD009850 | Pride

Transcription profiling of mouse macrophage responses to all-trans retinoic acid between wild type and NCoR-/- mice

Project description:Comparison of mouse macrophage responses to all-trans retinoic acid between wild-type and NCoR-/- mice.

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Apoplastic proteome upon powdery mildew Bgh challenge

Project description:In order to identify the key factors that are crucial for non-host resistance against Bgh and are secreted depending on SYP4-mediated membrane trafficking, we have performed apoplastic fluid preparation of a series of mutants defective in the SYP4 pathway. This project aims to identify proteomic profiles of these apoplastic fluids.

2019-01-11 | PXD009099 | Pride

Plasma membrane proteomics in the maize primary root elongation zone under water stress conditions

Project description:Maintenance of root growth is critical to plant adaptation to drought conditions. Previous work on the maize (Zea mays L.) primary root under water stress showed that cell elongation is maintained in the apical region of the growth zone but progressively inhibited further from the apex. These responses involve spatially differential and coordinated regulation of cellular growth processes, including modifications of both cell production rate and cell wall extensibility. As the interface between the cytoplasm and the apoplast (including the cell wall), the plasma membrane is likely to play major functions in the coordination of cell production and expansion. In addition, plasma membrane proteins may be involved in solute uptake for osmotic adjustment, pH regulation, ion homeostasis and other critical processes in roots growing under water-stressed conditions. Due to technical limitations, however, plasma membrane proteomic studies have not been reported for water-stressed tissues. Using a simplified method for enrichment of plasma membrane proteins, we compared the developmental distribution of plasma membrane proteins that are differentially regulated in the growth zone of well-watered and water-stressed roots.

2020-11-11 | PXD002027 | Pride

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