Browse
Submit Data
Databases
API
Help

Dataset Information

327 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Irradiation - The project is aimed at measuring DNA-repair enzymes and their change of concentration upon treatment with gamm-radiation, in six breast or breast-cancer derived cell lines. Two of the cell lines were analysed by shotgun proteomics to confirm the SRM data and identify alternative mechansims responsible for radio-resistance.

ABSTRACT: The project is aimed at measuring DNA-repair enzymes and their change of concentration upon treatment with gamm-radiation, in six breast or breast-cancer derived cell lines. Two of the cell lines were analysed by shotgun proteomics to confirm the SRM data and identify alternative mechansims responsible for radio-resistance.

INSTRUMENT(S): LTQ Orbitrap

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Epithelial Cell, Cell Culture, Fibroblast

DISEASE(S): Breast Cancer

SUBMITTER: Paolo Cifani

LAB HEAD: Peter James

PROVIDER: PXD000708 | Pride | 2016-01-13

REPOSITORIES: Pride

ACCESS DATA

Json Xml

Similar Datasets

A six-gene signature predicting breast cancer lung metastasis

Project description:The lungs are a frequent target of metastatic breast cancer cells, but the underlying molecular mechanisms are unclear. All existing data were obtained either using statistical association between gene expression measurements found in primary tumors and clinical outcome, or using experimentally derived signatures from mouse tumor models. Here, we describe a distinct approach that consists to utilize tissue surgically resected from lung metastatic lesions and compare their gene expression profiles with those from non-pulmonary sites, all coming from breast cancer patients. We demonstrate that the gene expression profiles of organ-specific metastatic lesions can be used to predict lung metastasis in breast cancer. We identified a set of 21 lung metastasis-associated genes. Using a cohort of 72 lymph node-negative breast cancer patients, we developed a six-gene prognostic classifier that discriminated breast primary cancers with a significantly higher risk of lung metastasis. We then validated the predictive ability of the six-gene signature in 3 independent cohorts of breast cancers consisting of a total of 721 patients. Finally, we demonstrated that the signature improves risk stratification independently of known standard clinical parameters and a previously established lung metastasis signature based on an experimental breast cancer metastasis model. Keywords: Disease state analysis

2008-08-15 | GSE11078 | GEO

Proteome characterization of Breast Cancer Cell lines

Project description:Protein profiling of 18 breast cancer cell lines in triplicates using TMT10-plex.

2020-07-04 | PXD013276 | JPOST Repository

VWCE inhibited the proliferation, migration, invasion, and chemoresistance of the breast cancer cell lines

Project description:Breast cancer remains a leading cause of cancer-related death, for which the majority of deaths result from metastases. Von Willebrand factor C and EGF domain (VWCE) is a member of the Von Willebrand factor (VWF) gene family; however, its function, regulatory mechanism, and clinical value in breast cancer remain unclear. In the present study, we sought to elucidate the role of VWCE in breast cancer metastasis. We examined the expression of VWCE in breast cancer tissues and normal control tissues of 50 breast cancer patients. We found that VWCE expression was downregulated in breast cancer cells and tissues compared to normal breast epithelial cells or the adjacent normal tissues. To explore the role of VWCE in human breast cancer development, we introduced a VWCE-overexpressing or control lentiviral vector into the breast cancer MDA-MB-453 and MDA-MB-231 lines in vitro. The overexpression of VWCE inhibited the proliferation, migration, invasion, and chemoresistance of the breast cancer cell lines. More import

2020-09-29 | PXD021733 | iProX

Identification of Key Genes and Pathways Associated With Irradiation in Breast Cancer Tissue and Breast Cancer Cell Lines.

Project description: Not available

| S-EPMC7346585 | biostudies-literature

Effect of actein on the growth of the MDA-MB-453 breast cancer cell lines as a function of time and concentration.

Project description:Previous studies indicate that the triterpene glycoside actein from the herb black cohosh inhibits growth of human breast cancer cells. This study seeks to identify genes altered in human breast cancer cells by treatment with actein, using gene expression analysis. We treated MDA-MB-453 human breast cancer cells with actein at 2 doses, 20 or 40 μg/mL, for 6 or 24 h. We identified 5 genes that were activated after each of the treatments that are known to play a role in cellular responses to diverse stresses, including the DNA damage and unfolded protein responses. In addition, four genes that mediate the integrated stress response (ISR), including activating transcription factor 4, were induced under at least one of the 4 treatment conditions. We used hierarchical clustering to define clusters comprising patterns of gene expression. Two ISR genes, activating transcription factor 3 (ATF3) and DNA damage- inducible transcript 3, and lipid biosynthetic genes were activated after exposure to actein at 40 μg/mL for 6 h, whereas the cell cycle genes cyclin E2 and cell division cycle 25A were repressed. Our results suggest that actein induces 2 phases of the ISR, the survival phase and the apoptotic phase, depending on the dose and duration of treatment. We confirmed the results of gene expression analysis with real-time RT-PCR for 18 selected genes and Western blot analysis for ATF3. Since actein activated transcription factors that enhance apoptosis, and repressed cell cycle genes, it may be useful in the prevention and therapy of breast cancer. Keywords: expression,time course, dose response

2009-04-15 | GSE7848 | GEO

Breast Cell Lines: Experimental vs. Mixed Reference

Project description:Transcriptional profiling of breast cell lines comparing breast cell line mixed reference with individual breast cell lines. Goal was to characterize breast cell line subtypes.

2009-10-10 | GSE18496 | GEO

A panel of breast cell lines

Project description:RNA expression patterns of breast cell lines were compared with a breast cell line mixed reference.

2013-02-22 | GSE44552 | GEO

Breast Cancer Cell Lines

Project description:Gene expression analysis on growing breast cancer cell lines. Keywords: cancer cell line profiles

2005-11-07 | GSE3156 | GEO

Mutation Screening of 1,237 Cancer Genes across Six Model Cell Lines of Basal-Like Breast Cancer.

Project description: Not available

| S-EPMC4684399 | biostudies-literature

RIME against TRPS1 in breast cancer cell lines

Project description:Identification of TRPS1 interactors in MCF7, T47D and ZR75-1 breast cancer cell lines.