Proteomics,Multiomics

Dataset Information

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TAF10 interacts with GATA1 transcription factor and controls mouse erythropoiesis

ABSTRACT: The stoichiometries of TFIID and SAGA (TAF10-containing complexes) have been quantified in mouse and human erythroid cells. TAF10 immunoprecipitations (IPs) have been carried out in erythroid cells at different stages of differentiation and development followed by quantitative mass spectrometry (MS). Interactions of TFIID and SAGA with several transcription factors and specifically with GATA-1 have been identified. GATA-1 immunoprecipitation (IP) in MEL cells also identified the reverse interactions with subunits of the TFIID and SAGA after MS analysis.

OTHER RELATED OMICS DATASETS IN: PRJNA281703

INSTRUMENT(S): LTQ Orbitrap, LTQ

ORGANISM(S): Homo Sapiens (human) Mus Musculus (mouse)

TISSUE(S): Mel Cell, Erythrocyte, B Cell, Hepatocyte, Liver, Fetal Cell Line

DISEASE(S): Polycystic Kidney,Acute Leukemia

SUBMITTER: Petros Papadopoulos  

LAB HEAD: Frank Grosveld

PROVIDER: PXD000729 | Pride | 2015-04-21

REPOSITORIES: Pride

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Publications

The ordered assembly of a functional preinitiation complex (PIC), composed of general transcription factors (GTFs), is a prerequisite for the transcription of protein-coding genes by RNA polymerase II. TFIID, comprised of the TATA binding protein (TBP) and 13 TBP-associated factors (TAFs), is the GTF that is thought to recognize the promoter sequences allowing site-specific PIC assembly. Transcriptional cofactors, such as SAGA, are also necessary for tightly regulated transcription initiation. T  ...[more]