Proteomics,Multiomics

Dataset Information

0

Lactate racemase proteins MALDI TOF/TOF


ABSTRACT: This project reports the characterization of the lactate racemase system in Lactobacillus plantarum. The lactate racemase (LarA) is a nickel-dependent enzyme. The apo-enzyme (apoLarA) is activated by a single nickel-containing maturation protein (LarE) that requires preactivation by two other accessory proteins (LarC which is a fusion between LarC1 and LarcC2 and LarB). Data submitted here identify the purified forms of Lar proteins.

OTHER RELATED OMICS DATASETS IN: PRJNA186636

INSTRUMENT(S): 4800 Proteomics Analyzer

ORGANISM(S): Lactobacillus Plantarum

SUBMITTER: Pascal Hols  

LAB HEAD: MORSOMME Pierre

PROVIDER: PXD000775 | Pride | 2014-04-02

REPOSITORIES: Pride

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Publications

Lactate racemase is a nickel-dependent enzyme activated by a widespread maturation system.

Desguin Benoît B   Goffin Philippe P   Viaene Eric E   Kleerebezem Michiel M   Martin-Diaconescu Vlad V   Maroney Michael J MJ   Declercq Jean-Paul JP   Soumillion Patrice P   Hols Pascal P  

Nature communications 20140407


Racemases catalyse the inversion of stereochemistry in biological molecules, giving the organism the ability to use both isomers. Among them, lactate racemase remains unexplored due to its intrinsic instability and lack of molecular characterization. Here we determine the genetic basis of lactate racemization in Lactobacillus plantarum. We show that, unexpectedly, the racemase is a nickel-dependent enzyme with a novel α/β fold. In addition, we decipher the process leading to an active enzyme, wh  ...[more]

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