Effects of Campylobacter jejuni CJIE1 prophage on protein expression experiment 4

ABSTRACT: Temperate bacteriophages (prophages) have recently been demonstrated in Campylobacter jejuni. However, what they do there is largely unknown. In the series of studies that are the subject of these submissions we have investigated the relative expression levels of proteins in C. jejuni isolates that differ in the presence or absence of the CJIE1 prophage. At the time of the initial investigations whole genome sequence data were not available for the isolates used, though DNA microarray data indicated that the isolates were very closely related. The overall project was carried out through four separate experiments. Previous work in the scientific literature indicated that growth on medium lacking blood but containing sodium deoxycholate induced the expression of at least some proteins associated with virulence and provided data thought to be of relevance to the virulence of the bacterium. The second set of experiments (experiment 2) compared protein expression in 4-plex iTRAQ experiments using two isolates grown on medium with and without 0.1% sodium deoxycholate to further explore the effects of the CJIE1 prophage, if any, on protein expression. Results from that experiment indicated that there was an unexpectedly large bile response, and that there were differences in expression from the first experiment that may be attributable to the presence of blood in the culture medium in the first experiment, compared with the use of medium without blood in the second experiment. Experiment 3 was therefore designed to compare protein expression of the CJIE1-carrying isolate 00-2425 after growth on medium containing blood, without blood, and without blood but with bile salts. In experiment 4 we grew isolate 00-2426, which lacks prophage CJIE1, on the same four media. The goals were to determine if the differences in protein expression (detection) were similar to those in isolate 00-2425 and if there were any differences that could be attributed to the presence or absence of the CJIE1 prophage.

OTHER RELATED OMICS DATASETS IN: PRJNA213842 PRJNA213841 PRJNA211797 PRJNA214465

INSTRUMENT(S):

ORGANISM(S): Campylobacter Jejuni Subsp. Jejuni

SUBMITTER: Clifford Clark  

LAB HEAD: Garrett R. Westmacott

PROVIDER: PXD000801 | Pride | 2014-07-31

REPOSITORIES: Pride