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Breast cancer tumors - System-wide Clinical Proteomics of Breast Cancer Reveals Global Remodeling of Tissue Homeostasis

ABSTRACT: The project analyzed 88 breast cancer clinical samples, including lymph node negative and positive primary tumors, lymph node metastases, and healthy tissue as control. All samples were combined with a super-SILAC mix that served as an internal standard for quantification.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Breast Invasive Ductal Carcinoma Cell, Epithelial Cell

DISEASE(S): Breast Cancer

SUBMITTER: Tamar Geiger

LAB HEAD: Tamar Geiger

PROVIDER: PXD000815 | Pride | 2016-01-25

REPOSITORIES: Pride

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System-wide Clinical Proteomics of Breast Cancer Reveals Global Remodeling of Tissue Homeostasis.

Pozniak Yair Y Balint-Lahat Nora N Rudolph Jan Daniel JD Lindskog Cecilia C Katzir Rotem R Avivi Camilla C Pontén Fredrik F Ruppin Eytan E Barshack Iris I Geiger Tamar T

Cell systems 20160303 3

The genomic and transcriptomic landscapes of breast cancer have been extensively studied, but the proteomes of breast tumors are far less characterized. Here, we use high-resolution, high-accuracy mass spectrometry to perform a deep analysis of luminal-type breast cancer progression using clinical breast samples from primary tumors, matched lymph node metastases, and healthy breast epithelia. We used a super-SILAC mix to quantify over 10,000 proteins with high accuracy, enabling us to identify k ...[more]

PMID: 27135363

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