Dataset Information

SIRT_mutant interactome - Comparative interactomes of SIRT6 and SIRTimplication of functional links to aging

ABSTRACT: In the present study, we identified SIRT6 (S56Y)- and SIRT7 (H187Y)-interacting proteins, and compared their interactomes to investigate functional links.
Extra primary submitter: Namgyu Lee, Department of Life Sciences, Pohang University of Science and Technology, Pohang, 790-784, Republic of Korea

INSTRUMENT(S): LTQ Orbitrap Velos

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Keratinocyte

SUBMITTER: Dae-Kyum Kim

LAB HEAD: Kwan Yong Choi

PROVIDER: PXD000850 | Pride | 2014-04-28

REPOSITORIES: Pride

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Comparative interactomes of SIRT6 and SIRT7: Implication of functional links to aging.

Lee Namgyu N Kim Dae-Kyum DK Kim Eung-Sam ES Park Sung Jin SJ Kwon Jung-Hee JH Shin Jihye J Park Seon-Min SM Moon Young Ho YH Wang Hee Jung HJ Gho Yong Song YS Choi Kwan Yong KY

Proteomics 20140528 13-14

Sirtuins are NAD(+) -dependent deacetylases that regulate a range of cellular processes. Although diverse functions of sirtuins have been proposed, those functions of SIRT6 and SIRT7 that are mediated by their interacting proteins remain elusive. In the present study, we identified SIRT6- and SIRT7-interacting proteins, and compared their interactomes to investigate functional links. Our interactomes revealed 136 interacting proteins for SIRT6 and 233 for SIRT7 while confirming seven and 111 pro ...[more]

PMID: 24782448

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Project description:Background:; Yersinia outer protein (Yop) H is a secreted virulence factor of Yersinia enterocolitica which inhibits phagocytosis of Y. enterocolitica and promotes virulence of Y. enterocolitica (Ye) in mice. The aim of this study was to address whether and how YopH affects the innate immune response against Ye in mice. Results:; For this purpose mice were infected with wild type Ye (pYV+) or a YopH-deficient Ye mutant strain (DyopH). CD11b+ cells were isolated from infected spleen and subjected to gene expression analysis using microarrays. Despite attenuation of DyopH in vivo, by variation of infection doses we were able to achieve conditions that allow comparison of gene expression in pYV+ and DyopH infections at either comparable infection courses or splenic bacterial burden. Gene expression analysis provided evidence that expression levels of several immune response genes including IFN-g and IL-6 are high after pYV+ infection but low after sublethal DyopH infection. In line with these findings, infection of IFN-gR-/- and IL-6-/- mice with pYV+ or DyopH revealed that these cytokines are dispensable for control of DyopH, but not pYV+ infection. Consistently, in bacteria killing assays with BMM in vitro, stimulation of BMM with IFN-g is required for killing of pYV+ but not DyopH. Conclusion:; In conclusion, this data suggest that IFN-g counteracts YopH-mediated virulence mechanisms of Ye which in Ye wild type infection contribute to evasion of the innate immune response including killing by macrophages. Experiment Overall Design: In this study microarray analyses were performed to define differences in gene expression of cells associated with innate immune response (CD11b+ cells) after infection of mice with a sublethal and lethal infection with wildtype Yersinia enterocolitica compared to uninfected mice. In addition, we wanted to investigate whether differences in gene expression can be defined which are due to the virulence factor YopH. Moroeover, we were interested whether gene expression pattern of sublethal and lethal infected mice are different. Allover all five samples were compared. Number of replicates 1.

			Action	DRS
	SIRT6_MUT_1.raw	Raw
	SIRT6_MUT_2.raw	Raw
	SIRT6_MUT_3.raw	Raw
	SIRT6_S56Y.zip	Other
	SIRT7_H187Y.zip	Other