Ontology highlight
ABSTRACT:
INSTRUMENT(S): LTQ Orbitrap
ORGANISM(S): Saccharomyces Cerevisiae (baker's Yeast)
SUBMITTER: Jagat Adhikari
LAB HEAD: Michael C. Fitzgerald
PROVIDER: PXD000860 | Pride | 2014-07-31
REPOSITORIES: Pride
Tran Duc T DT Adhikari Jagat J Fitzgerald Michael C MC
Molecular & cellular proteomics : MCP 20140416 7
Described here is a quantitative mass spectrometry-based proteomics method for the large-scale thermodynamic analysis of protein-ligand binding interactions. The methodology utilizes a chemical modification strategy termed, Stability of Proteins from Rates of Oxidation (SPROX), in combination with a Stable Isotope Labeling with Amino Acids in Cell Culture (SILAC) approach to compare the equilibrium folding/unfolding properties of proteins in the absence and presence of target ligands. The method ...[more]