Large-scale phosphoproteome analysis in seedling leaves of Brachypodium distachyon L

ABSTRACT: In this study, we carried out the first large-scale phosphoproteome analysis of seedling leaves in Brachypodium accession Bd21 using TiO2 microcolumns combined with liquid chromatography-tandem mass spectrometry (LC-MS/MS) and MaxQuant software. A total, 1,470 phosphorylation sites in 950 phosphoproteins were identified. Of the 950 phosphoproteins identified, 127 contained 3 to 8 phosphorylation sites. The phosphoproteins and phosphosites identified in our study expanded our knowledge of protein phosphorylation modification in plants, especially in monocot.

INSTRUMENT(S):

ORGANISM(S): Brachypodium Distachyon (purple False Brome) (trachynia Distachya)

TISSUE(S): Plant Cell, Leaf

DISEASE(S): Disease Free

SUBMITTER: Dong-Wen Lv  

LAB HEAD: Yue-Ming Yan

PROVIDER: PXD000868 | Pride | 2016-07-09

REPOSITORIES: Pride