Ontology highlight
ABSTRACT:
INSTRUMENT(S): LTQ
ORGANISM(S): Cyprinus Carpio (common Carp)
TISSUE(S): Spermatozoon, Sperm
SUBMITTER: Mariola Dietrich
LAB HEAD: Mariola A. Dietrich
PROVIDER: PXD000877 | Pride | 2014-10-13
REPOSITORIES: Pride
Dietrich Mariola A MA Arnold Georg J GJ Fröhlich Thomas T Ciereszko Andrzej A
Comparative biochemistry and physiology. Part D, Genomics & proteomics 20140928
Using a combination of protein fractionation by one-dimensional gel electrophoresis and high performance liquid chromatography-electrospray ionization tandem mass spectrometry, we identified 348 proteins in carp spermatozoa, most of which were for the first time identified in fish. Dynein, tubulin, HSP90, HSP70, HSP60, adenosylhomocysteinase, NKEF-B, brain type creatine kinase, mitochondrial ATP synthase, and valosin containing enzyme represent high abundance proteins in carp spermatozoa. These ...[more]