Reproducible Automated Phosphopeptide Enrichment using Magnetic TiO2 and Ti-IMAC

ABSTRACT: We describe the application of magnetic TiO2 and Ti-IMAC for uniform automated phosphopeptide enrichment. Combining hyper-porous magnetic microspheres with a magnetic particle-handling robot enables rapid (45 minutes), reproducible (r2 = 0.80) and high-fidelity (> 90% purity) phosphopeptide purification in a 96-well format.

INSTRUMENT(S):

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Epithelial Cell Of Pancreas, Pancreas

DISEASE(S): Pancreatic Ductal Adenocarcinoma

SUBMITTER: Christopher Tape  

LAB HEAD: Claus Jørgensen

PROVIDER: PXD000892 | Pride | 2014-09-22

REPOSITORIES: Pride