Proteomics

Dataset Information

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Reproducible Automated Phosphopeptide Enrichment using Magnetic TiO2 and Ti-IMAC

ABSTRACT: We describe the application of magnetic TiO2 and Ti-IMAC for uniform automated phosphopeptide enrichment. Combining hyper-porous magnetic microspheres with a magnetic particle-handling robot enables rapid (45 minutes), reproducible (r2 = 0.80) and high-fidelity (> 90% purity) phosphopeptide purification in a 96-well format.

INSTRUMENT(S): LTQ Orbitrap Velos, Q Exactive

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Epithelial Cell Of Pancreas, Pancreas

DISEASE(S): Pancreatic Ductal Adenocarcinoma

SUBMITTER: Christopher Tape  

LAB HEAD: Claus Jørgensen

PROVIDER: PXD000892 | Pride | 2014-09-22

REPOSITORIES: Pride

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Publications

Reproducible, comprehensive phosphopeptide enrichment is essential for studying phosphorylation-regulated processes. Here, we describe the application of hyper-porous magnetic TiO2 and Ti-IMAC microspheres for uniform automated phosphopeptide enrichment. Combining magnetic microspheres with a magnetic particle-handling robot enables rapid (45 min), reproducible (r2 ≥ 0.80) and high-fidelity (>90% purity) phosphopeptide purification in a 96-well format. Automated phosphopeptide enrichment demonst  ...[more]