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Human hnRNP H1 knockdown proteomics

ABSTRACT: HNRNP1 was characterized in a multi-dimensional study involving RNAi knockdown of the protein in HeLa cells. Label-free proteomics was used to characterize protein expression changes.

INSTRUMENT(S): LTQ Orbitrap Velos

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Permanent Cell Line Cell

DISEASE(S): Cervix Carcinoma

SUBMITTER: Christine Vogel

LAB HEAD: Christine Vogel

PROVIDER: PXD000895 | Pride | 2016-01-25

REPOSITORIES: Pride

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High-throughput analyses of hnRNP H1 dissects its multi-functional aspect.

Uren Philip J PJ Bahrami-Samani Emad E de Araujo Patricia Rosa PR Vogel Christine C Qiao Mei M Burns Suzanne C SC Smith Andrew D AD Penalva Luiz O F LO

RNA biology 20160113 4

hnRNPs are polyvalent RNA binding proteins that have been implicated in a range of regulatory roles including splicing, mRNA decay, translation, and miRNA metabolism. A variety of genome wide studies have taken advantage of methods like CLIP and RIP to identify the targets and binding sites of RNA binding proteins. However, due to the complex nature of RNA-binding proteins, these studies are incomplete without assays that characterize the impact of RBP binding on mRNA target expression. Here we ...[more]

PMID: 26760575

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