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Protein Redox Dynamics During Light-to-Dark Transitions in Cyanobacteria and Impacts Due to Nutrient Limitation

ABSTRACT: Protein Redox Dynamics During Light-to-Dark Transitions in Cyanobacteria and Impacts Due to Nutrient Limitation using chemical proteomics

INSTRUMENT(S): LTQ Orbitrap, LTQ Orbitrap Velos

ORGANISM(S): Synechococcus Sp. (strain Atcc 27264 / Pcc 7002 / Pr-6) (agmenellum Quadruplicatum)

TISSUE(S): Prokaryotic Cell, Cell Culture

SUBMITTER: Matthew Monroe

LAB HEAD: Richard D. Smith

PROVIDER: PXD000897 | Pride | 2014-08-29

REPOSITORIES: Pride

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Characterization of protein redox dynamics induced during light-to-dark transitions and nutrient limitation in cyanobacteria.

Ansong Charles C Sadler Natalie C NC Hill Eric A EA Lewis Michael P MP Zink Erika M EM Smith Richard D RD Beliaev Alexander S AS Konopka Allan E AE Wright Aaron T AT

Frontiers in microbiology 20140703

Protein redox chemistry constitutes a major void in knowledge pertaining to photoautotrophic system regulation and signaling processes. We have employed a chemical biology approach to analyze redox sensitive proteins in live Synechococcus sp. PCC 7002 cells in both light and dark periods, and to understand how cellular redox balance is disrupted during nutrient perturbation. The present work identified 300 putative redox-sensitive proteins that are involved in the generation of reductant, macrom ...[more]

PMID: 25071738

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