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Proteomic signatures of the Pacific oyster Crassostrea gigas cellular response to Ostreid herpes virus -1 (OsHV-1) infection: a metabolic response.

ABSTRACT: To better understand the pathogenesis of OsHV-1 and to determine which cell pathways might be affected during OsHV-1 infection, we used an experimental infection in the Pacific oyster Crassostrea gigas by intra-muscular injection with a low or high load of OsHV-1. Animals were sampled 2 days post-injection (dpi) which corresponds to the incubation period required for OsHV1 to initiate an intense replication phase. Twenty-five abundant proteins linked to infection process were identified using a two-dimensional electrophoresis (2-DE) proteomic approach.

INSTRUMENT(S): Bruker Daltonics HCT Series

ORGANISM(S): Crassostrea Gigas (pacific Oyster) (crassostrea Angulata)

TISSUE(S): Whole Body

SUBMITTER: E Com

LAB HEAD: Charles Pineau

PROVIDER: PXD000905 | Pride | 2014-07-10

REPOSITORIES: Pride

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Proteomic signatures of the oyster metabolic response to herpesvirus OsHV-1 μVar infection.

Corporeau Charlotte C Tamayo David D Pernet Fabrice F Quéré Claudie C Madec Stéphanie S

Journal of proteomics 20140705

Pacific oyster Crassostrea gigas were inoculated with OsHV-1 at low load (control) or high load (challenged) to better understand the pathogenesis of ostreid herpesvirus 1 (OsHV-1 μVar) and to determine which metabolic pathways might be affected during infection. Animals were sampled for proteomic analysis two days post-injection, at the same time as OsHV-1 initiated an intense replication phase in challenged oysters. Twenty-five abundant protein spots that showed a marked change in accumulated ...[more]

PMID: 25009143

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Physiological change under OsHV-1 contamination in Pacific oyster Crassostrea gigas through massive mortality events on fields

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2013-06-28 | E-GEOD-46249 | biostudies-arrayexpress

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