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Phosphoproteomic analysis of TCR signaling with inhibitors

ABSTRACT: MAPK-Erk and calcium-Calcineurin pathways are major signaling pathways activated during TCR signal transduction. We investigated the effect of these signaling pathway with the use of selective inhibitors of Mek and calcineurin.

INSTRUMENT(S): QSTAR

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Primary Cell, T Cell

SUBMITTER: Atsushi Hatano

LAB HEAD: Keiichi I. Nakayama

PROVIDER: PXD000972 | Pride | 2022-02-22

REPOSITORIES: Pride

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Phosphoproteomics analyses show subnetwork systems in T-cell receptor signaling.

Hatano Atsushi A Matsumoto Masaki M Nakayama Keiichi I KI

Genes to cells : devoted to molecular & cellular mechanisms 20160907 10

A key issue in the study of signal transduction is how multiple signaling pathways are systematically integrated into the cell. We have now performed multiple phosphoproteomics analyses focused on the dynamics of the T-cell receptor (TCR) signaling network and its subsystem mediated by the Ca<sup>2+</sup> signaling pathway. Integration of these phosphoproteomics data sets and extraction of components of the TCR signaling network dependent on Ca<sup>2+</sup> signaling showed unexpected phosphoryl ...[more]

PMID: 27600695

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