Proteomics

Dataset Information

73

Phosphoproteome of Hepatitis C Virus infected Huh7.5.1 cells


ABSTRACT: This project enriched and identified phosphoproteins in human hepatocarcinoma 7.5.1 cell line (Huh7.5.1) upon Hepatitis C virus (HCV) infection.

INSTRUMENT(S): LTQ Orbitrap

ORGANISM(S): Homo sapiens   Recombinant Hepatitis C virus J6/JFH1-J6  

TISSUE(S): Cell Culture

DISEASE(S): Not Available

SUBMITTER: Ming-Jiun Yu  

LAB HEAD: Ming-Jiun Yu

PROVIDER: PXD000988 | Pride | 2016-07-08

REPOSITORIES: pride

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Publications

Phosphoproteomics Identified an NS5A Phosphorylation Site Involved in Hepatitis C Virus Replication.

Chong Weng Man WM   Hsu Shih-Chin SC   Kao Wei-Ting WT   Lo Chieh-Wen CW   Lee Kuan-Ying KY   Shao Jheng-Syuan JS   Chen Yi-Hung YH   Chang Justin J   Chen Steve S-L SS   Yu Ming-Jiun MJ  

The Journal of biological chemistry 20151223 8


The non-structural protein 5A (NS5A) is a hepatitis C virus (HCV) protein indispensable for the viral life cycle. Many prior papers have pinpointed several serine residues in the low complexity sequence I region of NS5A responsible for NS5A phosphorylation; however, the functions of specific phosphorylation sites remained obscure. Using phosphoproteomics, we identified three phosphorylation sites (serines 222, 235, and 238) in the NS5A low complexity sequence I region. Reporter virus and replico  ...[more]

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