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SWATH Analysis of Yeast Proteome over Time in Response to Osmotic Stress

ABSTRACT: SWATH analysis of Yeast Proteome over time in response to Osmotic Stress. We sampled cell cultures in biological triplicates at six time points following the application of osmotic stress and acquired single injection DIA datasets on a high-resolution 5600 tripleTOF instrument operated in SWATH mode. Proteins were quantified by the targeted extraction and signal integration from the SWATH-MS datasets of peak groups representing proteotypic peptides for specific yeast proteins.

INSTRUMENT(S): TripleTOF 5600, TSQ Quantum

ORGANISM(S): Saccharomyces Cerevisiae (baker's Yeast)

SUBMITTER: Lucia Espona Pernas

LAB HEAD: Ruedi Aebersold

PROVIDER: PXD001010 | Pride | 2015-01-22

REPOSITORIES: Pride

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Reproducible and consistent quantification of the Saccharomyces cerevisiae proteome by SWATH-mass spectrometry.

Selevsek Nathalie N Chang Ching-Yun CY Gillet Ludovic C LC Navarro Pedro P Bernhardt Oliver M OM Reiter Lukas L Cheng Lin-Yang LY Vitek Olga O Aebersold Ruedi R

Molecular & cellular proteomics : MCP 20150105 3

Targeted mass spectrometry by selected reaction monitoring (S/MRM) has proven to be a suitable technique for the consistent and reproducible quantification of proteins across multiple biological samples and a wide dynamic range. This performance profile is an important prerequisite for systems biology and biomedical research. However, the method is limited to the measurements of a few hundred peptides per LC-MS analysis. Recently, we introduced SWATH-MS, a combination of data independent acquisi ...[more]

PMID: 25561506

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