Dataset Information

Proteomic analyses of embryogenic tissues in Picea balfouriana treated by 6-BAP

ABSTRACT: In this study, iTRAQ technology was applied to characterize the genes and proteins that are differentially expressed among tissues under the influence of different levels of 6-BAP(2.5 μM, 3.6 μM and 5 μM). There were two biological and three technological replicates for each treatment. Two biological replicates from the 2.5 μM treatment were labeled with reagents 115 and 121, while iTRAQ tags 117 and 119 were used to label two biological replicates from the 3.6 μM treatment, and two biological replicates from the 5 μM treatment were labeled with reagents 116 and 118.

INSTRUMENT(S): TripleTOF 5600

ORGANISM(S): Picea Likiangensis Var. Rubescens

TISSUE(S): Cell Culture

SUBMITTER: Qingfen Li

LAB HEAD: Junhui Wang

PROVIDER: PXD001022 | Pride | 2014-09-23

REPOSITORIES: Pride

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Publications

Transcriptomic and proteomic analyses of embryogenic tissues in Picea balfouriana treated with 6-benzylaminopurine.

Li Qingfen Q Zhang Shougong S Wang Junhui J

Physiologia plantarum 20141021 1

The cytokinin 6-benzylaminopurine (6-BAP) influences the embryogenic capacity of the tissues of Picea balfouriana during long subculture (after 3 months). Tissues that proliferate in 3.6 and 5 µM 6-BAP exhibit the highest and lowest embryogenic capacity, respectively, generating 113 ± 6 and 23 ± 3 mature embryos per 100 mg of tissue. In this study, a comparative transcriptomic and proteomic approach was applied to characterize the genes and proteins that are differentially expressed among tissue ...[more]

PMID: 25200684

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Project description:Polycyclic aromatic hydrocarbons (PAHs) are ubiquitous in the environment as components of fossil fuels and by-products of combustion. These multi-ring chemicals differentially activate the Aryl Hydrocarbon Receptor (AHR) in a structurally-dependent manner, and induce toxicity via both AHR-dependent and -independent mechanisms. PAH exposure is known to induce developmental malformations in zebrafish embryos, and recent studies have shown cardiac toxicity induced by compounds with low AHR affinity. Unraveling the potentially diverse molecular mechanisms of PAH toxicity is essential for understanding the hazard posed by complex PAH mixtures present in the environment. We analyzed transcriptional responses to PAH exposure in zebrafish embryos exposed to benz(a)anthracene (BAA), dibenzothiophene (DBT) and pyrene (PYR) at a concentration that induces developmental malformations by 120 hours post-fertilization (hpf). Whole genome microarray analysis of mRNA expression at 24 and 48 hpf identified genes that were differentially regulated over time and in response to the three PAH structures. PAH body burden was analyzed at these time points using GC-MS, and demonstrated differences in PAH uptake into the embryos. This was important for discerning dose-related differences from those that represented unique molecular mechanisms. While BAA misregulated the smallest number of transcripts, it caused strong induction of cyp1a and other genes known to be downstream of the AHR, which were not induced by the other two PAHs. Analysis of functional roles of misregulated genes and their predicted regulatory transcription factors also distinguished the BAA response from regulatory networks disrupted by DBT and PYR exposure. These results indicate that systems approaches can be used to classify the toxicity of PAHs based on the networks perturbed following exposure and may provide a path for unraveling the toxicity of complex PAH mixtures. Gene expression was measured in zebrafish embryos after exposure to PAHs. Embryos were batch-exposed in groups of 40 to 25 μM BAA, 25 μM DBT, 25 μM PYR or 1% DMSO vehicle control starting at 6 hpf and collected at 24 or 48 hpf. Four independent biological replicates were prepared for each treatment. The reference was a pool of zebrafish embryos exposed to DMSO control until 24 and 48 hpf.

Dataset Information

Proteomic analyses of embryogenic tissues in Picea balfouriana treated by 6-BAP

Publications

Transcriptomic and proteomic analyses of embryogenic tissues in Picea balfouriana treated with 6-benzylaminopurine.

Similar Datasets

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