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Systematic evaluation of quantotypic peptides for targeted analysis of the human kinome

ABSTRACT: In targeted proteomics it is critical that peptides are not only proteotypic, but also accurately represent the level of the protein (quantotypic). Numerous approaches are used to identify proteotypic peptides, but quantotypic properties are rarely assessed. Here, we show that measuring ratios of proteotypic peptides across biological samples can be used to empirically identify peptides with good quantotypic properties, and use this to identify quantotypic peptides for 21% of the human kinome.

INSTRUMENT(S): LTQ Orbitrap Velos, TSQ Vantage

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Pancreatic Ductal Cell, Cell Culture

DISEASE(S): Pancreas Adenocarcinoma

SUBMITTER: Jonathan Worboys

LAB HEAD: Claus Jørgensen

PROVIDER: PXD001026 | Pride | 2014-08-08

REPOSITORIES: Pride

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Systematic evaluation of quantotypic peptides for targeted analysis of the human kinome.

Worboys Jonathan D JD Sinclair John J Yuan Yinyin Y Jørgensen Claus C

Nature methods 20140824 10

In targeted proteomics it is critical that peptides are not only proteotypic but also accurately represent the level of the protein (quantotypic). Numerous approaches are used to identify proteotypic peptides, but quantotypic properties are rarely assessed. We show that measuring ratios of proteotypic peptides across biological samples can be used to empirically identify peptides with good quantotypic properties. We applied this technique to identify quantotypic peptides for 21% of the human kin ...[more]

PMID: 25152083

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