Dataset Information
In-depth phosphoproteomic profiling of mouse microglia BV-2 cell line
Ontology highlight
ABSTRACT:
INSTRUMENT(S): Q Exactive
ORGANISM(S): Mus Musculus (mouse)
TISSUE(S): Microglial Cell
SUBMITTER: 
Dohyun Han
LAB HEAD: Dohyun Han
PROVIDER: PXD001033 | Pride | 2014-10-15
REPOSITORIES: Pride
Publications
Integrated approach using multistep enzyme digestion, TiO2 enrichment, and database search for in-depth phosphoproteomic profiling.
Proteomics 20141008 2-3
Protein phosphorylation is a major PTM that regulates important cell signaling mechanisms. In-depth phosphoproteomic analysis provides a method of examining this complex interplay, yielding a mechanistic understanding of the cellular processes and pathogenesis of various diseases. However, the analysis of protein phosphorylation is challenging, due to the low concentration of phosphoproteins in highly complex mixtures and the high variability of phosphorylation sites. Thus, typical phosphoproteo ...[more]
