Ontology highlight
ABSTRACT:
INSTRUMENT(S): Agilent instrument model
ORGANISM(S): Homo Sapiens (human)
TISSUE(S): Blood Serum
DISEASE(S): Cervix Carcinoma
SUBMITTER: Alexander Boychenko
LAB HEAD: Rainer Bischoff
PROVIDER: PXD001055 | Pride | 2014-10-06
REPOSITORIES: Pride
Boichenko Alexander P AP Govorukhina Natalia N Klip Harry G HG van der Zee A G J AG Güzel Coşkun C Luider Theo M TM Bischoff Rainer R
Journal of proteome research 20140929 11
We developed a discovery-validation mass-spectrometry-based pipeline to identify a set of proteins that are regulated in serum of patients with cervical intraepithelial neoplasia (CIN) and squamous cell cervical cancer using iTRAQ, label-free shotgun, and targeted mass-spectrometric quantification. In the discovery stage we used a "pooling" strategy for the comparative analysis of immunodepleted serum and revealed 15 up- and 26 down-regulated proteins in patients with early- (CES) and late-stage ...[more]