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Label free IMS-MSE protein quantification for plant Golgi protein localisation and abundance

ABSTRACT: Golgi resident proteins in Arabidopsis thaliana are challenging to quantify since the abundance of this organelle is relatively low within the cell. In this study an organelle fractionation approach, targeting the Golgi apparatus, was combined with a label free quantitative MS, data-independent acquisition (DIA) method employing ion mobility separation known as LC-IMS-MSE, to simultaneously localise proteins to the Golgi apparatus and assess their relative quantity. In total 33 proteins were localised to Golgi apparatus and 102 Golgi localised proteins were quantified.

INSTRUMENT(S): Synapt MS

ORGANISM(S): Arabidopsis Thaliana (mouse-ear Cress)

TISSUE(S): Callus Culture

SUBMITTER: Pavel Shliaha

LAB HEAD: Kathryn Susan Lilley

PROVIDER: PXD001056 | Pride | 2016-07-07

REPOSITORIES: Pride

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Label-free protein quantification for plant Golgi protein localization and abundance.

Nikolovski Nino N Shliaha Pavel V PV Gatto Laurent L Dupree Paul P Lilley Kathryn S KS

Plant physiology 20140813 2

The proteomic composition of the Arabidopsis (Arabidopsis thaliana) Golgi apparatus is currently reasonably well documented; however, little is known about the relative abundances between different proteins within this compartment. Accurate quantitative information of Golgi resident proteins is of great importance: it facilitates a better understanding of the biochemical processes that take place within this organelle, especially those of different polysaccharide synthesis pathways. Golgi reside ...[more]

PMID: 25122472

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