Proteomics

Dataset Information

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Fe-IMAC column based phospho enrichment

ABSTRACT: Fe-IMAC columns for robust and reproducible phosphopeptide ernichment, comparison to TiO2 batch and Ti-IMAC tip enrichment, large scale phosphoproteomics coupling Fe-IMAC column pre-enrichment to subsequent hSAX separation

INSTRUMENT(S): LTQ Orbitrap Velos

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Permanent Cell Line Cell, Epithelial Cell

SUBMITTER: Benjamin Ruprecht  

LAB HEAD: Prof. Dr. Bernhard Kuster

PROVIDER: PXD001060 | Pride | 2015-08-19

REPOSITORIES: Pride

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Publications

Comprehensive and reproducible phosphopeptide enrichment using iron immobilized metal ion affinity chromatography (Fe-IMAC) columns.

Ruprecht Benjamin B   Koch Heiner H   Medard Guillaume G   Mundt Max M   Kuster Bernhard B   Lemeer Simone S  

Molecular & cellular proteomics : MCP 20141113 1

Advances in phosphopeptide enrichment methods enable the identification of thousands of phosphopeptides from complex samples. Current offline enrichment approaches using TiO(2), Ti, and Fe immobilized metal ion affinity chromatography (IMAC) material in batch or microtip format are widely used, but they suffer from irreproducibility and compromised selectivity. To address these shortcomings, we revisited the merits of performing phosphopeptide enrichment in an HPLC column format. We found that F  ...[more]