Substrate Trapping Proteomics Reveals Targets of the βTrCP2/FBXW11 Ubiquitin Ligase

ABSTRACT: We inhibited the proteosome to ‘trap’ ubiquitylated substrates on the SCFFBXW11 E3 complex. Comparative mass spectrometry analysis of immunopurified FBXW11 protein complexes before and after proteosome inhibition revealed known and putatively novel substrates.

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Permanent Cell Line Cell, Cell Culture

SUBMITTER: Dennis Goldfarb  

LAB HEAD: Michael Ben Major

PROVIDER: PXD001062 | Pride | 2014-10-24

REPOSITORIES: Pride