Proteomics

Dataset Information

597

Proteomics analysis of rough endoplasmic reticulum in pancreatic beta cells


ABSTRACT: Pancreatic beta cells have well-developed endoplasmic reticulum (ER) to accommodate for the massive production and secretion of insulin. ER homeostasis is vital for normal beta cell function. Perturbation of ER homeostasis contributes to beta cell dysfunction in both type 1 and type 2 diabetes. To systematically identify the molecular machinery responsible for proinsulin biogenesis and maintenance of beta cell ER homeostasis, a widely used mouse pancreatic beta cell line, MIN6 cell was used to purify rough ER. Two different purification schemes were utilized. In each experiment, the ER pellets were solubilized and analyzed by one dimensional SDS-PAGE coupled with HPLC-MS/MS. A total of 1467 proteins were identified in three experiments with ≥95% confidence, among which 1117 proteins were found in at least two separate experiments. Gene ontology analysis revealed a comprehensive profile of known and novel players responsible for proinsulin biogenesis and ER homeostasis. This dataset defines a molecular environment in the ER for proinsulin synthesis, folding and export and laid a solid foundation for further characterizations of altered ER homeostasis under diabetes-causing conditions.

INSTRUMENT(S): QSTAR

ORGANISM(S): Mus musculus  

TISSUE(S): Cell Culture

DISEASE(S): Not Available

SUBMITTER: Xuequn Chen  

LAB HEAD: Xuequn Chen

PROVIDER: PXD001081 | Pride | 2015-04-15

REPOSITORIES: Pride

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Publications

Proteomics analysis of rough endoplasmic reticulum in pancreatic beta cells.

Lee Jin-sook JS   Wu Yanning Y   Schnepp Patricia P   Fang Jingye J   Zhang Xuebao X   Karnovsky Alla A   Woods James J   Stemmer Paul M PM   Liu Ming M   Zhang Kezhong K   Chen Xuequn X  

Proteomics 20150501 9


Pancreatic beta cells have well-developed ER to accommodate for the massive production and secretion of insulin. ER homeostasis is vital for normal beta cell function. Perturbation of ER homeostasis contributes to beta cell dysfunction in both type 1 and type 2 diabetes. To systematically identify the molecular machinery responsible for proinsulin biogenesis and maintenance of beta cell ER homeostasis, a widely used mouse pancreatic beta cell line, MIN6 cell was used to purify rough ER. Two diff  ...[more]

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