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Spike-in comparison - Data processing has major impact on the outcome of quantitative label-free LC-MS analysis

ABSTRACT: Synthetic peptides were spiked in at different levels in a digested cell lysate from Streptococcus pyogenes

INSTRUMENT(S): LTQ Orbitrap XL ETD

ORGANISM(S): Streptoccous Pyogenes

SUBMITTER: Fredrik Levander

LAB HEAD: Fredrik Levander

PROVIDER: PXD001091 | Pride | 2014-11-20

REPOSITORIES: Pride

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Data processing has major impact on the outcome of quantitative label-free LC-MS analysis.

Chawade Aakash A Sandin Marianne M Teleman Johan J Malmström Johan J Levander Fredrik F

Journal of proteome research 20141126 2

High-throughput multiplexed protein quantification using mass spectrometry is steadily increasing in popularity, with the two major techniques being data-dependent acquisition (DDA) and targeted acquisition using selected reaction monitoring (SRM). However, both techniques involve extensive data processing, which can be performed by a multitude of different software solutions. Analysis of quantitative LC-MS/MS data is mainly performed in three major steps: processing of raw data, normalization, ...[more]

PMID: 25407311

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