Proteomics

Dataset Information

Soybean Symbiosome Membrane Proteome

ABSTRACT: Symbiosome membranes (SM) and symbiosome space (SS) fractions were isolated from nitrogen fixing soybean root nodules and analyzed using non-gel proteomic techniques. Bicarbonate stripping and chloroform-methanol extraction of isolated SM were used to enrich for hydrophobic integral membrane proteins.. One-hundred and seventy-two proteins were identified as components of the SM, with an additional fifteen proteins identified from peripheral membrane and SS protein fractions. Proteins involved in a range of cellular processes such as metabolism, protein folding and targeting, signalling and transport were identified. These included a number of proteins previously localized to the SM, such as nodulin 26, an aquaglyceroporin. Transport classes identified include sulphate, calcium, peptide/dicarboxylate, nitrate and metal ion transporters. This proteome will provide a rich resource for the study of the legume-rhizobium symbiosis.

INSTRUMENT(S):

ORGANISM(S): Glycine Max

TISSUE(S): Root Nodule

SUBMITTER: Victoria Clarke  

LAB HEAD: Penny Smith

PROVIDER: PXD001132 | Pride | 2015-08-12

REPOSITORIES: Pride

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Action DRS
20110827_J1D (J1D).mzid_20110827_J1D_(J1D).pride.mgf.gz Mzid
20110827_J1D (J1D).pride.mztab.gz Mztab
20110827_J1DJ1D.mzid.gz Mzid
20110827_J1DJ1D.mzid_20110827_J1D_J1D.MGF Mzid
20110827_J1_A (J1A).mzid_20110827_J1_A_(J1A).pride.mgf.gz Mzid
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