Proteomics

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Hendra virus infection of bat (Pteropus alecto) and human cells


ABSTRACT: SILAC labeled human kidney cells (293 cells) or bat kidney cells (PakiT03cells)were infected with Hendra virus for 8 or 24 hours and compared to uninfected control cells. Protein identification and quantitation relied on a combination of Uniprot lists of proteins and Proteomics Informed by Transcriptomics (PIT) analysis whereby RNA extracted from the same samples was deep sequenced and the sequencing data was used to construct mRNA from which possible ORFS were inferred and used as a search space by MaxQuant.

INSTRUMENT(S): LTQ Orbitrap Velos

ORGANISM(S): Homo Sapiens (human) Pteropus Alecto (black Flying Fox) Hendra Virus (isolate Horse/autralia/hendra/1994)

TISSUE(S): Epithelial Cell, Kidney

DISEASE(S): (-)ssrna Virus Infectious Disease

SUBMITTER: David Matthews  

LAB HEAD: David A Matthews

PROVIDER: PXD001165 | Pride | 2014-11-05

REPOSITORIES: Pride

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