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Proteomics analysis of virulent and avirulent strains of mycobacterium

ABSTRACT: label free quantification analysis was performed on four strains of mycobacterium tuberculosis. The four strains are H37Rv, H37Ra, BND and JAL. The purpose of this study is to identify strain specific proteome expression pattern among the four strains.

INSTRUMENT(S): LTQ Orbitrap Velos

ORGANISM(S): Mycobacterium Tuberculosis

SUBMITTER: gagan jhingan

LAB HEAD: Vinay Kumar Nandicoori

PROVIDER: PXD001188 | Pride | 2016-05-10

REPOSITORIES: Pride

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Comparative Proteomic Analyses of Avirulent, Virulent, and Clinical Strains of Mycobacterium tuberculosis Identify Strain-specific Patterns.

Jhingan Gagan Deep GD Kumari Sangeeta S Jamwal Shilpa V SV Kalam Haroon H Arora Divya D Jain Neharika N Kumaar Lakshmi Krishna LK Samal Areejit A Rao Kanury V S KVS Kumar Dhiraj D Nandicoori Vinay Kumar VK

The Journal of biological chemistry 20160505 27

Mycobacterium tuberculosis is an adaptable intracellular pathogen, existing in both dormant as well as active disease-causing states. Here, we report systematic proteomic analyses of four strains, H37Ra, H37Rv, and clinical isolates BND and JAL, to determine the differences in protein expression patterns that contribute to their virulence and drug resistance. Resolution of lysates of the four strains by liquid chromatography, coupled to mass spectrometry analysis, identified a total of 2161 prot ...[more]

PMID: 27151218

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