Dataset Information

Quantitative Phosphorylation analysis in human platelets after ADP stimulation

ABSTRACT: Human platelets were treated with 20 µM of ADP for 10, 30 and 60 s in triplicate. In a second experiment blood donations of three donors were split again in four fractions and one was treated with 20 µM of ADP and the second and third with 20 µM of ADP for 30 s and subsequently with 2 nM of Iloprost for further 30 s and 60 s.

INSTRUMENT(S): LTQ Orbitrap Velos, Q Exactive

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Blood Platelet, Platelet, Blood

SUBMITTER: Florian Beck

LAB HEAD: Florian Beck

PROVIDER: PXD001189 | Pride | 2017-03-14

REPOSITORIES: Pride

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Publications

Temporal quantitative phosphoproteomics of ADP stimulation reveals novel central nodes in platelet activation and inhibition.

Beck Florian F Geiger Jörg J Gambaryan Stepan S Solari Fiorella A FA Dell'Aica Margherita M Loroch Stefan S Mattheij Nadine J NJ Mindukshev Igor I Pötz Oliver O Jurk Kerstin K Burkhart Julia M JM Fufezan Christian C Heemskerk Johan W M JW Walter Ulrich U Zahedi René P RP Sickmann Albert A

Blood 20161109 2

Adenosine diphosphate (ADP) enhances platelet activation by virtually any other stimulant to complete aggregation. It binds specifically to the G-protein-coupled membrane receptors P2Y1 and P2Y12, stimulating intracellular signaling cascades, leading to integrin αIIbβ3 activation, a process antagonized by endothelial prostacyclin. P2Y12 inhibitors are among the most successful antiplatelet drugs, however, show remarkable variability in efficacy. We reasoned whether a more detailed molecular unde ...[more]

PMID: 28060719

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Project description:Dinoflagellates have evolved a nuclear organization unlike that of any other eukaryotic group. Recent studies find a predominance of post-transcriptional control of dinoflagellate gene expression. This study investigated regulation of the environmental stress response in the red tide dinoflagellate, Karenia brevis using an Agilent custom oligonucleotide microarray. K. brevis cultures were exposed to 5°C or 10°C heat shock, or three different sources of oxidative stress: 60 µM H2O2, 10 mM NaNO2, or 12 µM PbCl2 over acute time courses. Ribosomal genes, genes involved in RNA processing, translation, and chaperones were among the classes of genes consistently downregulated across treatments, although within these functional classes the same genes did not always respond to different stressors. Genes involved in the photosystem and mitochondrial and chloroplast ATP generation dominated the down-regulated genes. Heat shock and oxidative stress response genes were not induced under any treatment, even under conditions that resulted in decreased viability. We subsequently identified the presence of a trans-spliced leader sequence on many stress response gene transcripts, which suggests that they may be transcribed constitutively and their expression regulated at the level of translation. Cultures of were grown to mid-log phase. For each treatment, five replicate untreated control cultures and five replicate treated cultures were harvested at several time points following treatment. The following time courses were used: 5°C heat shock - 5, 15, 30, 60, and 240 min; 10°C heat shock - 60 min; 60 µM H2O2 - 5, 15, 30, 60, and 240 min; 10 mM NaNO2 -1, 4, and 7 hours; 12 µM PbCl2 -1, 4, and 7 hours. For each treatment, RNA was pooled from the controls and treated cultures at each timepoint. Two color arrays were then run comparing each the transcriptome at timepoint with the pooled control for that treatment. A technical dye swap array was run at each timepoint.

Dataset Information

Quantitative Phosphorylation analysis in human platelets after ADP stimulation

Publications

Temporal quantitative phosphoproteomics of ADP stimulation reveals novel central nodes in platelet activation and inhibition.

Similar Datasets

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