Proteomics

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Identification of long-lived proteins retained in cells undergoing repeated asymmetric divisions

ABSTRACT: To identify long-lived proteins that are retained in yeast mother cells, we combined a method developed in our lab with a pulse-chase protocol using total proteome analysis. Cells were initially grown in medium with stable heavy-isotope amino acids to label proteins. The cells were then switched into media containing light-isotope amino acids and cultured for an additional ~18 cell divisions with the MEP engaged. Finally, we determined whether any of the original heavy-labeled proteins were still present in the aged mother cells

INSTRUMENT(S): LTQ Orbitrap

ORGANISM(S): Saccharomyces Cerevisiae (baker's Yeast)

TISSUE(S): Diploid Cell

SUBMITTER: Nathaniel Thayer  

LAB HEAD: Daniel E. Gottschling

PROVIDER: PXD001251 | Pride | 2014-09-01

REPOSITORIES: Pride

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Identification of long-lived proteins retained in cells undergoing repeated asymmetric divisions.

Thayer Nathaniel H NH   Leverich Christina K CK   Fitzgibbon Matthew P MP   Nelson Zara W ZW   Henderson Kiersten A KA   Gafken Philip R PR   Hsu Jessica J JJ   Gottschling Daniel E DE  

Proceedings of the National Academy of Sciences of the United States of America 20140916 39

Long-lived proteins have been implicated in age-associated decline in metazoa, but they have only been identified in extracellular matrices or postmitotic cells. However, the aging process also occurs in dividing cells undergoing repeated asymmetric divisions. It was not clear whether long-lived proteins exist in asymmetrically dividing cells or whether they are involved in aging. Here we identify long-lived proteins in dividing cells during aging using the budding yeast, Saccharomyces cerevisia  ...[more]