Identification of long-lived proteins retained in cells undergoing repeated asymmetric divisions

ABSTRACT: To identify long-lived proteins that are retained in yeast mother cells, we combined a method developed in our lab with a pulse-chase protocol using total proteome analysis. Cells were initially grown in medium with stable heavy-isotope amino acids to label proteins. The cells were then switched into media containing light-isotope amino acids and cultured for an additional ~18 cell divisions with the MEP engaged. Finally, we determined whether any of the original heavy-labeled proteins were still present in the aged mother cells

INSTRUMENT(S):

ORGANISM(S): Saccharomyces Cerevisiae (baker's Yeast)

TISSUE(S): Diploid Cell

SUBMITTER: Nathaniel Thayer  

LAB HEAD: Daniel E. Gottschling

PROVIDER: PXD001251 | Pride | 2014-09-01

REPOSITORIES: Pride