Proteomics

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Rat Liver Phosphoproteome - An optimized platform for hydrophilic interaction chromatography-immobilized metal affinity chromatography enables deep coverage of the rat liver phosphoproteome

ABSTRACT: In depth analysis of rat liver phosphoproteome using optimized HILIC IMAC sample preparation

INSTRUMENT(S):

ORGANISM(S): Rattus Rattus (black Rat)

TISSUE(S): Liver

SUBMITTER: Roland Annan  

LAB HEAD: Roland S Annan

PROVIDER: PXD001372 | Pride | 2016-05-04

REPOSITORIES: Pride

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MaxQuant_output.zip Other
RatLiver_FASP_IMAC_0411run_16to24_90min_2-15and15-30_041513.raw Raw
RatLiver_FASP_IMAC_0411run_25to28_90min_2-15and15-30_041513.raw Raw
RatLiver_FASP_IMAC_0411run_29and30_90min_2-15and15-30_041513.raw Raw
RatLiver_FASP_IMAC_0411run_31and32_90min_2-15and15-30_041513.raw Raw
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An optimized platform for hydrophilic interaction chromatography-immobilized metal affinity chromatography enables deep coverage of the rat liver phosphoproteome.

Zappacosta Francesca F   Scott Gilbert F GF   Huddleston Michael J MJ   Annan Roland S RS  

Journal of proteome research 20150126 2

While analysis of the phosphoproteome has become an important component of understanding how cells function, it remains a nontrivial task in terms of the number of sample preparation steps and instrument time needed to achieve sufficient depth of coverage to produce meaningful results. We previously described a multidimensional method that uses hydrophilic interaction chromatography (HILIC) followed by Fe(3+) immobilized metal affinity chromatography (IMAC) to reduce complexity, improve selectiv  ...[more]

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