Human Transcription Factors LC-MSMS

ABSTRACT: How transcription factors (TFs), the ultimate targets and executors of cellular signalling pathways, are regulated via protein-protein interactions remains elusive. To systematically investigate the regulations and functions of human transcription factors, we performed proteomics studies of soluble and chromatin-associated complexes of 56 TFs in HEK293T cells, using tandem-affinity-purification followed by mass spectrometry (TAP/MS). We performed 214 purifications and identified 2,156 high-confident protein-protein interactions.

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Hek-293t Cell, Early Embryonic Cell

DISEASE(S): Disease Free

SUBMITTER: Xu Li  

LAB HEAD: Junjie Chen

PROVIDER: PXD001383 | Pride | 2015-04-07

REPOSITORIES: Pride