Proteomics

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Intensity-based label-free proteomics using a quadrupole orbitrap mass spectrometer

ABSTRACT: We present a unique data set for benchmarking label free quantitative proteomics using a quadrupole orbitrap mass spectrometer. Soluble Escherichia coli digest was spiked into a HeLa digest in four different concentrations, simulating protein expression differences. The data set, which is available online, provides a unique opportunity to test the proteomic platform (instrumentation and software) in its ability to perform intensity based label free quantification. We show that the presented combination of informatics and instrumentation produces high precision and quantification accuracy.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Homo Sapiens (human) Escherichia Coli

TISSUE(S): Cell Culture

SUBMITTER: Yishai Levin  

LAB HEAD: Yishai Levin

PROVIDER: PXD001385 | Pride | 2015-03-25

REPOSITORIES: Pride

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Publications

MS1-based label-free proteomics using a quadrupole orbitrap mass spectrometer.

Shalit Tali T   Elinger Dalia D   Savidor Alon A   Gabashvili Alexandra A   Levin Yishai Y  

Journal of proteome research 20150324 4

Presented is a data set for benchmarking MS1-based label-free quantitative proteomics using a quadrupole orbitrap mass spectrometer. Escherichia coli digest was spiked into a HeLa digest in four different concentrations, simulating protein expression differences in a background of an unchanged complex proteome. The data set provides a unique opportunity to evaluate the proteomic platform (instrumentation and software) in its ability to perform MS1-intensity-based label-free quantification. We sh  ...[more]