Protein Quantification and Identification from River Bank Grape, Vitis riparia: Comparing SDS-PAGE and FASP-GPF techniques for shotgun proteomic analysis.

ABSTRACT: The aim of this quantitative label-free shotgun proteomic experiment is to perform a comparative study of two different sample preparation methods which employ two different pre-fractionation techniques (SDS-PAGE and FASP-GPF) for use in shotgun proteomic analysis of Vitis riparia leaf material with concurrent label-free quantitation in order to optimise a technique that would facilitate identification of most number of proteins and produce useful biological information, when searched against the Vitis vinifera database.

INSTRUMENT(S):

ORGANISM(S): Vitis Riparia (frost Grape) (vitis Vulpina) Vitis Vinifera (grape)

TISSUE(S): Plant Cell, Leaf

SUBMITTER: Iniga George  

LAB HEAD: Paul A Haynes

PROVIDER: PXD001399 | Pride | 2018-04-13

REPOSITORIES: Pride