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Protein Quantification and Identification from River Bank Grape, Vitis riparia: Comparing SDS-PAGE and FASP-GPF techniques for shotgun proteomic analysis.

ABSTRACT: The aim of this quantitative label-free shotgun proteomic experiment is to perform a comparative study of two different sample preparation methods which employ two different pre-fractionation techniques (SDS-PAGE and FASP-GPF) for use in shotgun proteomic analysis of Vitis riparia leaf material with concurrent label-free quantitation in order to optimise a technique that would facilitate identification of most number of proteins and produce useful biological information, when searched against the Vitis vinifera database.

INSTRUMENT(S): LTQ Orbitrap Velos

ORGANISM(S): Vitis Riparia (frost Grape) (vitis Vulpina) Vitis Vinifera (grape)

TISSUE(S): Plant Cell, Leaf

SUBMITTER: Iniga George

LAB HEAD: Paul A Haynes

PROVIDER: PXD001399 | Pride | 2018-04-13

REPOSITORIES: Pride

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Protein identification and quantification from riverbank grape, Vitis riparia: Comparing SDS-PAGE and FASP-GPF techniques for shotgun proteomic analysis.

George Iniga S IS Fennell Anne Y AY Haynes Paul A PA

Proteomics 20150622 17

Protein sample preparation optimisation is critical for establishing reproducible high throughput proteomic analysis. In this study, two different fractionation sample preparation techniques (in-gel digestion and in-solution digestion) for shotgun proteomics were used to quantitatively compare proteins identified in Vitis riparia leaf samples. The total number of proteins and peptides identified were compared between filter aided sample preparation (FASP) coupled with gas phase fractionation (GP ...[more]

PMID: 25929842

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