Off-line high pH reversed-phase fractionation for in-depth phosphoproteomics

ABSTRACT: In this study we provide a detailed analysis of the physicochemical characteristics of phosphopeptides, which have been fractionated by off-line high pH chromatography (HpH) before subsequent titanium dioxide (TiO2) enrichment and LC-MS/MS analysis. Our results demonstrate that HpH is superior to standard strong-cation exchange (SCX) fractionation in total number of phosphopeptides detected when analyzing the same number of fractions by identical 70 minutes LC-MS/MS gradients

INSTRUMENT(S):

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Cell Culture, Fibroblast

SUBMITTER: Tanveer Batth  

LAB HEAD: Jesper V. Olsen

PROVIDER: PXD001404 | Pride | 2014-10-23

REPOSITORIES: Pride