Ancient proteins resolve the evolutionary history of Darwin's South American ungulates

ABSTRACT: The project aimed to characterize the collagen type I (COL1) sequences from Pleistocene Macrauchenia sp. and Toxodon sp. bone samples, and by comparison with existing COL1 sequences available from genomic sources establish the phylogenetic position of both extinct species. In order to resolve their phylogenetic position, COL1 was extracted from two Toxodon (samples MLP201204, MACN201212, York12, York13) and two Macrauchenia (samples MLP201212, MACN201202, York14, York15). In addition, modern and Pleistocene COL1 was extracted from additional species currently not present in available databases (Mylodon darwinii, Cyclopes didactylus, Hippopotamus amphibius, Tapirus terrestris) or from species for which COL1 sequences are available (Equus sp., Oryceropus afer). All extractions were performed at BioArCh, University of York (UK). Analyses took place on Bruker maXis HD (Macrauchenia sp., Toxodon sp., Equus sp.) and Thermo Scientific Hybrid Quadruopole-Orbitrap (Macrauchenia sp., Toxodon sp., Mylodon darwinii, Cyclopes didactylus, Hippopotamus amphibius, Tapirus terrestris, Oryceropus afer) platforms.

INSTRUMENT(S):

ORGANISM(S): Orycteropus Afer (aardvark) Equus Tapirus Terrestris (lowland Tapir) (brazilian Tapir) Cyclopes Didactylus (silky Anteater) (myrmecophaga Didactyla) Hippopotamus Amphibius (hippopotamus) Unidentified Mylodon Darwinii (giant Ground Sloth)

TISSUE(S): Skin, Bone

SUBMITTER: Frido Welker  

LAB HEAD: Matthew Collins

PROVIDER: PXD001411 | Pride | 2015-03-19

REPOSITORIES: Pride