Dataset Information

Ancient proteins resolve the evolutionary history of Darwin's South American ungulates

ABSTRACT: The project aimed to characterize the collagen type I (COL1) sequences from Pleistocene Macrauchenia sp. and Toxodon sp. bone samples, and by comparison with existing COL1 sequences available from genomic sources establish the phylogenetic position of both extinct species. In order to resolve their phylogenetic position, COL1 was extracted from two Toxodon (samples MLP201204, MACN201212, York12, York13) and two Macrauchenia (samples MLP201212, MACN201202, York14, York15). In addition, modern and Pleistocene COL1 was extracted from additional species currently not present in available databases (Mylodon darwinii, Cyclopes didactylus, Hippopotamus amphibius, Tapirus terrestris) or from species for which COL1 sequences are available (Equus sp., Oryceropus afer). All extractions were performed at BioArCh, University of York (UK). Analyses took place on Bruker maXis HD (Macrauchenia sp., Toxodon sp., Equus sp.) and Thermo Scientific Hybrid Quadruopole-Orbitrap (Macrauchenia sp., Toxodon sp., Mylodon darwinii, Cyclopes didactylus, Hippopotamus amphibius, Tapirus terrestris, Oryceropus afer) platforms.

INSTRUMENT(S): maXis, LTQ Orbitrap Velos, Q Exactive

ORGANISM(S): Orycteropus Afer (aardvark) Equus Tapirus Terrestris (lowland Tapir) (brazilian Tapir) Cyclopes Didactylus (silky Anteater) (myrmecophaga Didactyla) Hippopotamus Amphibius (hippopotamus) Unidentified Mylodon Darwinii (giant Ground Sloth)

TISSUE(S): Skin, Bone

SUBMITTER: Frido Welker

LAB HEAD: Matthew Collins

PROVIDER: PXD001411 | Pride | 2015-03-19

REPOSITORIES: Pride

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Publications

Ancient proteins resolve the evolutionary history of Darwin's South American ungulates.

Welker Frido F Collins Matthew J MJ Thomas Jessica A JA Wadsley Marc M Brace Selina S Cappellini Enrico E Turvey Samuel T ST Reguero Marcelo M Gelfo Javier N JN Kramarz Alejandro A Burger Joachim J Thomas-Oates Jane J Ashford David A DA Ashton Peter D PD Rowsell Keri K Porter Duncan M DM Kessler Benedikt B Fischer Roman R Baessmann Carsten C Kaspar Stephanie S Olsen Jesper V JV Kiley Patrick P Elliott James A JA Kelstrup Christian D CD Mullin Victoria V Hofreiter Michael M Willerslev Eske E Hublin Jean-Jacques JJ Orlando Ludovic L Barnes Ian I MacPhee Ross D E RD

Nature 20150318 7554

No large group of recently extinct placental mammals remains as evolutionarily cryptic as the approximately 280 genera grouped as 'South American native ungulates'. To Charles Darwin, who first collected their remains, they included perhaps the 'strangest animal[s] ever discovered'. Today, much like 180 years ago, it is no clearer whether they had one origin or several, arose before or after the Cretaceous/Palaeogene transition 66.2 million years ago, or are more likely to belong with the elepha ...[more]

PMID: 25799987

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Dataset Information

Ancient proteins resolve the evolutionary history of Darwin's South American ungulates

Publications

Ancient proteins resolve the evolutionary history of Darwin's South American ungulates.

Similar Datasets

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