Proteomics

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Characterization and quantification of chicken eggshell proteins during pivotal events of shell calcification

ABSTRACT: Gallus gallus avian eggshell is composed of 95% calcium carbonate on calcitic form and of 3.5% extracellular organic matrix (proteins, polysaccharides and proteoglycans). This highly ordered structure with a polycrystalline organization result of the control of mineral deposition by the organic matrix components in the lumen of the uterus. This interaction leads to the eggshell ultrastructure and consequently contributes to its resulting mechanical properties. This study used GeLC MS/MS analyses combined to label free quantitative analysis to identify and quantify matrix proteins at the pivotal step of the calcification process (amorphous calcium carbonate deposit, amorphous calcium carbonate transformation into calcite, large calcite crystal units and rapid growth phase). The study gave new insight on proteins playing crucial role in the biomineralization of the shell.

INSTRUMENT(S): LTQ Orbitrap Velos

ORGANISM(S): Gallus Gallus (chicken)

SUBMITTER: G HARICHAUX  

LAB HEAD: Joel GAUTRON

PROVIDER: PXD001450 | Pride | 2015-06-09

REPOSITORIES: Pride

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Publications

Quantitative proteomics provides new insights into chicken eggshell matrix protein functions during the primary events of mineralisation and the active calcification phase.

Marie Pauline P   Labas Valérie V   Brionne Aurélien A   Harichaux Grégoire G   Hennequet-Antier Christelle C   Rodriguez-Navarro Alejandro B AB   Nys Yves Y   Gautron Joël J  

Journal of proteomics 20150603

Eggshell is a bioceramic composed of 95% calcium carbonate mineral and 3.5% organic matrix. Its structural organisation is controlled by its organic matrix. We have used quantitative proteomics to study four key stages of shell mineralisation: 1) widespread deposition of amorphous calcium carbonate (ACC), 2) ACC transformation into crystalline calcite aggregates, 3) formation of larger calcite crystal units and 4) development of a columnar structure with preferential calcite crystal orientation.  ...[more]