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Label-free analysis of HCV-associated hepatic fibrosis

ABSTRACT: A differential label-fee proteomics approach was performed using 27 biopsies from patients with HCV-associated hepatic fibrosis. For statistical analysis the patients were grouped into a low and a high fibrosis group. The low fibrosis group contained 13 patients of fibrosis stages 0, 1 and 2, whereas the high fibrosis group contained 14 patients of fibrosis stages 3 and 4 (fibrosis stages according to Batts-Ludwig classification).

INSTRUMENT(S): LTQ Orbitrap Elite

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Hepatocyte, Liver

DISEASE(S): Hepatitis C

SUBMITTER: Thilo Bracht

LAB HEAD: Jun. Prof. Dr. Barbara Sitek

PROVIDER: PXD001474 | Pride | 2015-03-30

REPOSITORIES: Pride

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Analysis of disease-associated protein expression using quantitative proteomics—fibulin-5 is expressed in association with hepatic fibrosis.

Bracht Thilo T Schweinsberg Vincent V Trippler Martin M Kohl Michael M Ahrens Maike M Padden Juliet J Naboulsi Wael W Barkovits Katalin K Megger Dominik A DA Eisenacher Martin M Borchers Christoph H CH Schlaak Jörg F JF Hoffmann Andreas-Claudius AC Weber Frank F Baba Hideo A HA Meyer Helmut E HE Sitek Barbara B

Journal of proteome research 20150406 5

Hepatic fibrosis and cirrhosis are major health problems worldwide. Until now, highly invasive biopsy remains the diagnostic gold standard despite many disadvantages. To develop noninvasive diagnostic assays for the assessment of liver fibrosis, it is urgently necessary to identify molecules that are robustly expressed in association with the disease. We analyzed biopsied tissue samples from 95 patients with HBV/HCV-associated hepatic fibrosis using three different quantification methods. We per ...[more]

PMID: 25807371

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