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H.pylori, N.gonorrhoeae, E.coli LC-MS/MS

ABSTRACT: We have developed approach and algorithm to annotated mass-spectra database creation and further spectral based amino acid substitution search. Algorithm is based on a robust procedure of spectral angle comparison. We have shown applicability of the algorithm to large scale datasets of Helicobacter pylori, Neisseria gonorrhoeae and Escherichia coli

INSTRUMENT(S): maXis, TripleTOF 5600

ORGANISM(S): Helicobacter Pylori 26695-1 Helicobacter Pylori H13-1 Helicobacter Pylori E48 Escherichia Coli Neisseria Gonorrhoeae (strain Atcc 700825 / Fa 1090) Helicobacter Pylori A45 Helicobacter Pylori J99 (campylobacter Pylori J99) Neisseria Gonorrhoeae Escherichia Coli Str. K-12 Substr. Mg1655

SUBMITTER: Ilya Altukhov

LAB HEAD: Dmitry Alexeev

PROVIDER: PXD001481 | Pride | 2016-12-23

REPOSITORIES: Pride

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Large scale analysis of amino acid substitutions in bacterial proteomics.

Ischenko Dmitry D Alexeev Dmitry D Shitikov Egor E Kanygina Alexandra A Malakhova Maja M Kostryukova Elena E Larin Andrey A Kovalchuk Sergey S Pobeguts Olga O Butenko Ivan I Anikanov Nikolay N Altukhov Ilya I Ilina Elena E Govorun Vadim V

BMC bioinformatics 20161108 1

<h4>Background</h4>Proteomics of bacterial pathogens is a developing field exploring microbial physiology, gene expression and the complex interactions between bacteria and their hosts. One of the complications in proteomic approach is micro- and macro-heterogeneity of bacterial species, which makes it impossible to build a comprehensive database of bacterial genomes for identification, while most of the existing algorithms rely largely on genomic data.<h4>Results</h4>Here we present a large sca ...[more]

PMID: 27821049

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