Proteomics

Dataset Information

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Bacillus cereus proteomics (Intraproteome)


ABSTRACT: This project contributes to the intraproteomic comparison of Bacillus cereus wild-type and Bacillus cereus mutant devoided of the EntD protein grown in aerobiosis condition and harvested at the early exponential growth stage.

INSTRUMENT(S): LTQ Orbitrap

ORGANISM(S): Bacillus Cereus (strain Atcc 14579 / Dsm 31)

SUBMITTER: Jean ARMENGAUD  

LAB HEAD: Jean ARMENGAUD

PROVIDER: PXD001483 | Pride | 2016-06-29

REPOSITORIES: Pride

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Publications

Proteomics identifies Bacillus cereus EntD as a pivotal protein for the production of numerous virulence factors.

Omer Hélène H   Alpha-Bazin Béatrice B   Brunet Jean-Luc JL   Armengaud Jean J   Duport Catherine C  

Frontiers in microbiology 20151007


Bacillus cereus is a Gram-positive pathogen that causes a wide variety of diseases in humans. It secretes into the extracellular milieu proteins that may contribute directly or indirectly to its virulence. EntD is a novel exoprotein identified by proteogenomics of B. cereus ATCC 14579. We constructed a ΔentD mutant and analyzed the impact of entD disruption on the cellular proteome and exoproteome isolated from early, late, and stationary-phase cultures. We identified 308 and 79 proteins regulat  ...[more]

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