Yeast - phosphoproteome - Yeast PP4 Interacts with ATR Homolog Ddc2-Mec1 and Regulates Checkpoint Signaling
Ontology highlight
ABSTRACT:
INSTRUMENT(S): LTQ Orbitrap Velos
ORGANISM(S): Saccharomyces Cerevisiae (baker's Yeast)
TISSUE(S): Cell Culture
SUBMITTER: 
Ragna Sack
LAB HEAD: Susan M Gasser
PROVIDER: PXD001492 | Pride | 2015-01-12
REPOSITORIES: Pride
Publications
Yeast PP4 interacts with ATR homolog Ddc2-Mec1 and regulates checkpoint signaling.
Hustedt Nicole N Seeber Andrew A Sack Ragna R Tsai-Pflugfelder Monika M Bhullar Bhupinder B Vlaming Hanneke H van Leeuwen Fred F Guénolé Aude A van Attikum Haico H Srivas Rohith R Ideker Trey T Shimada Kenji K Gasser Susan M SM
Molecular cell 20141218 2
Mec1-Ddc2 (ATR-ATRIP) controls the DNA damage checkpoint and shows differential cell-cycle regulation in yeast. To find regulators of Mec1-Ddc2, we exploited a mec1 mutant that retains catalytic activity in G2 and recruitment to stalled replication forks, but which is compromised for the intra-S phase checkpoint. Two screens, one for spontaneous survivors and an E-MAP screen for synthetic growth effects, identified loss of PP4 phosphatase, pph3Δ and psy2Δ, as the strongest suppressors of mec1-10 ...[more]
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